Part:BBa_K123003:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQd0d0c1b83c18542d-partinfo-00000000-QINU

• jbrown2 ([http://2011.igem.org/Team:Caltech Caltech iGEM team 2011])

We ran the DNA sequence through the ExPASy translator with the following result: MAGMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAA YEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSP FLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESA KETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQA CRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSTG-RKRRNTRWPATCVR RTCGRLR--SNVLKKTLWRCL-PRTRWFLRCWTRNRRSCTLNTTRPVRSLKRL-WVC-PT WRTVNWFT-STGRNVFRVSLT-PCTTRFTCWNARGWKS--SVWFGVLWNIRVNCCSRRTC CWTVTRVNALKVWLKSSTCCWRPLLVSV--TYKVKSLFA-NLSSC-TLVFTPSCLLP-NL WKKKTTSTVFWTKSPTP-ST-WRKRV-PYNSSTSVWRSCC-SCLTSVTCLTKVWNTCTL- NAKTLFRCTTCCWKCWTRTVCTRRPLVVVRLLKKPTSLTWRPRVLPLLTLYKNTTSPAKR KVSRRPLPV It looks like there's an intron in the coding sequence. This should be the correct sequence: >gi|306921463|dbj|BAJ17811.1| estrogen receptor 1 [synthetic construct] MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANA QVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYT VREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFK RSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEV GSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLM AKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASV EETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV Update: September 27, 2011: We sequenced most of the gene. It seems the team codon optimized and submitted a different sequence in silico than what the physical DNA actually is. Here is the sequence: ATGACTCTGCACACTAAAGCATCTGGCATGGCGCTGCTGCATCAGATCCA GGGCAACGAACTGGAGCCGCTGAATCGCCCGCAGCTGAAGATCCCACTGG AACGTCCGCTGGGCGAGGTCTACCTGGATTCCAGCAAGCCAGCTGTATAT AACTATCCGGAAGGTGCAGCGTACGAATTTAACGCTGCGGCTGCGGCCAA CGCCCAAGTTTACGGCCAAACTGGTCTGCCGTATGGCCCTGGTAGCGAAG CAGCCGCTTTCGGTAGCAACGGCCTGGGTGGTTTTCCGCCTCTGAACTCT GTATCTCCGTCCCCGCTGATGCTGCTGCACCCTCCGCCGCAGCTGTCTCC GTTCCTGCAACCGCACGGCCAGCAGGTGCCGTACTACCTGNNNNNNNNNN NNNNNNNNNNNNNNGTGCGTGAGGCTGGTCCACCGGCGTTCTATCGTCCG AATAGCGACAACCGTCGCCAGGGCGGTCGTGAACGCCTGGCTTCCACCAA CGACAAAGGCTCTATGGCTATGGAATCTGCGAAAGAAACCCGTTACTGTG CAGTGTGTAACGACTATGCATCCGGTTACCATTACGGCGTTTGGAGCTGT GAGGGCTGCAAAGCGTTCTTCAAGCGCTCCATTCAGGGTCACAACGATTA CATGTGCCCGGCCACCAATCAGTGCACGATCGACAAAAACCGTCGTAAAT CCTGCCAGGCATGTCGTCTGCGTAAATGCTATGAAGTTGGCATGATGAAA GGTGGTATTCGTAAAGATCGCCGCGGTGGCCGTATGCTGAAACACAAACG TCAGCGCGATGACGGTGAAGGTCGTGGTGAAGTTGGTTCTACCGGCGATA TGCGCGCAGCTAATCTGTGGCCTTCCCCACTGATGATCAAACGTTCTAAG AAAAACAGCCTGGCGCTGTCCCTGACCGCCGACCAGATGGTATCCGCACT GCTGGACGCAGAACCGCCGATTCTGTACAGCGAGTACGACCCAACGCGCC CGTTTAGCGAGGCAAGCATGATGGGTCTGCTGACCAACCTGGCTGATCGT GAACTGGTCCATATGATCAACTGGGCGAAACGCGTTCCGGGTTTCGTAGA CCTGACTCTGCACGACCAGGTCCACCTGCTGGAATGTGCATGGCTGGAGA TCCTGATGATCGGCCTGGTTTGGCGTTCCATGGAACACCCGGGTAAGCTG CTGTTTGCCCCGAACCTGCTGCTGGACCGTAACCAGGGTAANTGCGTGGA AGGCATGGTTGAAATTTTCGATATGCTGCTGGCCACCTCTAGCCGTTTCC GCATGATGAACCTGCAAGGTGAAGAGTTCGTGTGCCTGAAATCCATCATT CTGCTGAACTCCGGCGTGTATACGTTCCTGAGCTCTACCCTGAAATCTCT GGAGGAAAAAGATCACATCCACCGTGTGCTGGACAAAATCACTGATACCC TGATTCACCTGATGGCAAAAGCTGGCCTGACCCTGCAACAGCAGCACCAG CGTCTGGCGCAGCTGCTGCTGATCCTGTCTCACATCCGCCACATGAGCAA TAAGGGTATGGAACATCTGTACTCTATGAAATGTAAAAACGTTGTTCCGC TGTACGATCTGCTGCTGGAAATGCTGGACGCCCACCGTCTGCATGCGCCG ACCAGCCGTGGCGGTGCTTCCGTTGAAGAAACTGATCAGTCTCATCTGGC GACCGCTGGCTCCACCTCTTCCCACTCTCTGCAAAAATATTACATTACTG GTGAAGCGGAAGGCTTCCCGGCGACCGTAACCGGTTAA

;

UNIQd0d0c1b83c18542d-partinfo-00000002-QINU